NOTE: the best way to figure out what you need to work on is to do 'Ctrl+F' and search for your name. Hit 'enter' to find the next instance. However, some tasks say 'All' so your name won't show up specificallly - Thanks, Dr. B
----- this assumes I haven't misspelled your name!
Lab Safety mentors - Charina, Andee
Please fill out your weekly schedule here:- ALL
Use the MentorLabLog on GDocs/VDSstaff for a Lab Notebook for Wet Lab tasks - ALL
Put new dates on Daily Schedule (i.e. take last year's items for each week and put the new dates for this year there) - Andee
. Dr. B already did Week 0, Week 1, Week 2
Send out FRI Facebook link -Andee
Send out FRI LinkeIn link - whichever one of you has a LinkeIn profile
Journal Club
Set up journal club page for Summer 2015 -Nikki DONE
make schedule of presenters -Nikki (in progress)
Research Presentations Schedule - Nikki
- Mentors to update - but not yet (need some research first)
- will be on the Journal Club page since that will also have the list of Journal Club presentations
Lab Safety (Monday)
LAB SAFETY MENTORS: Andee, Charina
Update Lab Safety page in Wikispaces with names of each students and table, link from left hand menu.
See Lab Safety Folder in GDrive/VDSstaff
- Practice & Plan Lab Safety Walk Throughs.
OH 201 Lab Safety Walk through – Charina and Nikki to lead (or Andee depending on timing)
See One, Do One, Teach One
Print Pink & Blue & Orange Sign Off Sheet for Site Specific training (Autoclave, Centrifuge)
in GDocs/Misc/
--- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Blue.docx -
--- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx -
--- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx -
Show these to the students during safety training
Afix these to the Big Centrifuge (in DIY lab), and the Sonicator (in our lab)
Wait to Afix these to the FPLC (? not yet), Autoclave (? - not yet),
Summer Plasmids
- designate Summer Plasmids for students to Transform and make more of this summer - Andee
Hours for Students
Update Hours spreadsheet in GDocs - Andee Charina
Tally weekly hours from sign in sheet and enter into spreadsheet on VDSStaff - Andee or Nikki
Create Research Pages for each student (OR SHOULD WE JUST DO DROPBOX FOLDERS?)
- (use Research Pages 2014 as a guide)
Targets
- make Target Selection form (copy from Summ14 - or make fresh) - once we know which targets are options
Teaching Modules (in class brief activities – 5 slides, 1 activity, 2 slides)
PCR, RE Digest, Cloning, etc.
- Charina
- Nikki
- Andee
Lab Tech Tasks (John G) - see Purchasing sheet on GDrive for items
What do I do with a package that is delivered to lab?: Open, figure out if it is VDS, show to John G. if there, Label appropriately, Place in proper storage, Place receipt in Dr. B's pigeon hole by the lab notebooks and let him know it has arrived and is stored properly.
LAB WORK
Blue Juice for protein gels - (need DTT - check first) -
Rinse the 'clean' collirollers in nanopure water, transfer to a clean bottle, label - then Autoclave (use autoclave tape) - make students do
Divide up colirrolers in to 6 rollers each in sterile tubes of (600 ul tubes) - make a full ~ 80 tube rack of these - make students do
Autoclave Nanopure water, label, store in Cubby. Make 2 x 500ml. - this is for 1st Lab to make 30% EtOH - make students do
SOLUTIONS RECIPES & INSTRUCTIONS:
(also see the Solutions&Dilutions Spreadsheet on GDocs for help with calculations)
Make LB - 2Liters - and autoclave Make 5 M NaCl - 500 ml in water, sterile filter into a bottle, label, store at Room Temp
Make Kanamycin- stocks 50ug/ul store in -20degC, Make 30 tubes of 500 ul each. Sterile filter with syringe filterbefore aliquoting into tubes. Label. Store in -20degC
Make IPTG - 1 M IPTG stock to make. Make 30 tubes of this with 500 ul in each. Label. Store in -20degC
Make Lysozyme - 20 aliquots of 500 ul at 50 mg/ml in 1.7 ml tubes. Label. Store in -20degC
Make TCEP - make ~50 x 100ul aliquots of 500 mM aliquots medium size centrifuge tubes. Label, put in a box and Store at Room Temp in cubby.
Make PMSF - 30 x 500 ul aliquots of 10mM in Isopropanol 1.7 ml tubes. Label. store in -20degC
Make Imidazole - 1M stock make in beaker of 500 ml of water, then bottle top sterile filter it into a bottle, store at Room Temp
Lysis Buffer for Sonication (make 500 ml)
(see GDocs 'Solutions&Dilutions' in /Misc folder for calculations)
Tris 100 mM
NaCl 300 mM
Imidazole 30 mM
pH 8.0 w/ NaOH
label, store at 4degC
NEW Wash and Elution Buffers and Storage Buffer(see GDocs 'Solutions&Dilutions' in /Misc folder for calculations)
Wash Buffer
Tris 100 mM
NaCl 300 mM
Imidazole 30 mM
pH 8.0 w/ NaOH
Elution Buffer
Tris 100 mM
NaCl 300 mM
Imidazole 300 mM
pH 8.0 w/ NaOH
FPLC column buffer - tris Also 'storage' buffer
Tris 50 mM
NaCl 150 mM
pH 8.0 w/ NaOH
Then filter sterilize
Then degass**
6X SDS Page Buffer from 5 Prime Protocol
30 ml of glycerol – add first so that you can see the volume (viscous)
(In a separate tube) - 30 ml of Nanopure water
--- 1.635 g Tris powder
--- 3 g SDS poweder
--- 0.3 g Bromophenol blue
----2.3136 g DTT
Mix contents of second tube into tube with glycerol
Add some more Nanopure water to 50 ml total final volume
Printer Installation
Login as Mentor, Start Menu,
Devices And printers
Add New Printer
Add "local" printer
Create New Port --> standard TCP pathway
Enter IP 128.82.195.186
Printer Name Lexmark C543
Add Printer (if can't find printer do Window Update)
For installation of a different Printer (such as Dell) change the IP address and Printer Name
MENTOR TASKS
NOTE: the best way to figure out what you need to work on is to do 'Ctrl+F' and search for your name. Hit 'enter' to find the next instance. However, some tasks say 'All' so your name won't show up specificallly - Thanks, Dr. B----- this assumes I haven't misspelled your name!
Lab Safety mentors - Charina, Andee
Please fill out your weekly schedule here:- ALL
Use the MentorLabLog on GDocs/VDSstaff for a Lab Notebook for Wet Lab tasks - ALL
Put new dates on Daily Schedule (i.e. take last year's items for each week and put the new dates for this year there) - Andee
. Dr. B already did Week 0, Week 1, Week 2
Send out FRI Facebook link -Andee
Send out FRI LinkeIn link - whichever one of you has a LinkeIn profile
Journal Club
Set up journal club page for Summer 2015 -Nikki DONEmake schedule of presenters -Nikki (in progress)
Research Presentations Schedule - Nikki
- Mentors to update - but not yet (need some research first)- will be on the Journal Club page since that will also have the list of Journal Club presentations
Lab Safety (Monday)
LAB SAFETY MENTORS: Andee, CharinaUpdate Lab Safety page in Wikispaces with names of each students and table, link from left hand menu.
See Lab Safety Folder in GDrive/VDSstaff
- Practice & Plan Lab Safety Walk Throughs.
OH 201 Lab Safety Walk through – Charina and Nikki to lead (or Andee depending on timing)
See One, Do One, Teach One
Print Pink & Blue & Orange Sign Off Sheet for Site Specific training (Autoclave, Centrifuge)
in GDocs/Misc/
--- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Blue.docx -
--- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx -
--- SignOffSheet_SiteSpecific_Centrifuge_Autoclave_etc_Pink.docx -
Show these to the students during safety training
Afix these to the Big Centrifuge (in DIY lab), and the Sonicator (in our lab)
Wait to Afix these to the FPLC (? not yet), Autoclave (? - not yet),
Summer Plasmids
- designate Summer Plasmids for students to Transform and make more of this summer - Andee
Hours for Students
Update Hours spreadsheet in GDocs - Andee Charina
Tally weekly hours from sign in sheet and enter into spreadsheet on VDSStaff - Andee or Nikki
Create Research Pages for each student (OR SHOULD WE JUST DO DROPBOX FOLDERS?)
- (use Research Pages 2014 as a guide)
Targets
- make Target Selection form (copy from Summ14 - or make fresh) - once we know which targets are options
Teaching Modules (in class brief activities – 5 slides, 1 activity, 2 slides)
PCR, RE Digest, Cloning, etc.
- Charina
- Nikki
- Andee
Lab Tech Tasks (John G) - see Purchasing sheet on GDrive for items
https://drive.google.com/open?id=19gXV3i-WeguSKluGbEH21akQqgE--S4UhNEUKmzalx4&authuser=0What do I do with a package that is delivered to lab?:
Open, figure out if it is VDS, show to John G. if there, Label appropriately, Place in proper storage, Place receipt in Dr. B's pigeon hole by the lab notebooks and let him know it has arrived and is stored properly.
LAB WORK
Blue Juice for protein gels - (need DTT - check first) -
Rinse the 'clean' collirollers in nanopure water, transfer to a clean bottle, label - then Autoclave (use autoclave tape) - make students do
Divide up colirrolers in to 6 rollers each in sterile tubes of (600 ul tubes) - make a full ~ 80 tube rack of these - make students do
Autoclave Nanopure water, label, store in Cubby. Make 2 x 500ml. - this is for 1st Lab to make 30% EtOH - make students do
SOLUTIONS RECIPES & INSTRUCTIONS:
(also see the Solutions&Dilutions Spreadsheet on GDocs for help with calculations)Make LB - 2Liters - and autoclave
Make 5 M NaCl - 500 ml in water, sterile filter into a bottle, label, store at Room Temp
Make Kanamycin- stocks 50ug/ul store in -20degC, Make 30 tubes of 500 ul each. Sterile filter with syringe filterbefore aliquoting into tubes. Label. Store in -20degC
Make IPTG - 1 M IPTG stock to make. Make 30 tubes of this with 500 ul in each. Label. Store in -20degC
Make Lysozyme - 20 aliquots of 500 ul at 50 mg/ml in 1.7 ml tubes. Label. Store in -20degC
Make TCEP - make ~50 x 100ul aliquots of 500 mM aliquots medium size centrifuge tubes. Label, put in a box and Store at Room Temp in cubby.
Make PMSF - 30 x 500 ul aliquots of 10mM in Isopropanol 1.7 ml tubes. Label. store in -20degC
Make Imidazole - 1M stock make in beaker of 500 ml of water, then bottle top sterile filter it into a bottle, store at Room Temp
Lysis Buffer for Sonication (make 500 ml)
(see GDocs 'Solutions&Dilutions' in /Misc folder for calculations)Tris 100 mM
NaCl 300 mM
Imidazole 30 mM
pH 8.0 w/ NaOH
label, store at 4degC
NEW Wash and Elution Buffers and Storage Buffer(see GDocs 'Solutions&Dilutions' in /Misc folder for calculations)
Wash Buffer
Tris 100 mM
NaCl 300 mM
Imidazole 30 mM
pH 8.0 w/ NaOH
Elution Buffer
Tris 100 mM
NaCl 300 mM
Imidazole 300 mM
pH 8.0 w/ NaOH
FPLC column buffer - tris Also 'storage' buffer
Tris 50 mM
NaCl 150 mM
pH 8.0 w/ NaOH
Then filter sterilize
Then degass**
6X SDS Page Buffer from 5 Prime Protocol
30 ml of glycerol – add first so that you can see the volume (viscous)(In a separate tube) - 30 ml of Nanopure water
--- 1.635 g Tris powder
--- 3 g SDS poweder
--- 0.3 g Bromophenol blue
----2.3136 g DTT
Mix contents of second tube into tube with glycerol
Add some more Nanopure water to 50 ml total final volume
Printer Installation
- Login as Mentor, Start Menu,
- Devices And printers
- Add New Printer
- Add "local" printer
- Create New Port --> standard TCP pathway
- Enter IP 128.82.195.186
- Printer Name Lexmark C543
- Add Printer (if can't find printer do Window Update)
For installation of a different Printer (such as Dell) change the IP address and Printer NamePrinter Name: Lexmark C543 IP: 128.83.195.186
Printer Name: Dell IP: 129.116.152.49
COMPLETED STUFF++++++++++++++++++++++++++++++++++++++++++++++++++++++++
MentorsSp15 (last semester's mentors page)
M 3-4, T 4-5, F 2-3